Rosmarinic Add and Other Phenolic Acids in Hairy Roots of Hyssopus officinalis

Ewa Kochan3, H alina W ysokinskab, A leksander Chmiel3 and B arbara G rabiasb a L aboratory of Biosynthesis of Drugs, Medical University, Muszynskiego 1, 90-151 Lodz, Poland b D epartm ent of Biology and Pharm aceutical Botany, Medical University, Muszynskiego 1, 90-151 Lodz, Poland Z. N aturforsch. 54c, 11-16 (1999); received July 15/September 9, 1998 H yssopus officinalis, Agrobacterium rhizogenes, Transformed Roots, Phenolic Acids, Rosm arinic Acid H airy roots of H yssopus officinalis L. were induced by infection of petioles with Agrobacte­ rium rhizogenes LBA 9402 and studied for production of phenolic acids, especially rosm arinic acid (R A ). The highest content of rosmarinic acid (about 6 % of dry weight) was obtained in hairy roots grown in G am borg’s B5 liquid medium containing 10% (w/v) sucrose. The level was at least 60% higher than those found in callus, cell suspension culture and roots of one-year-old field grown plants. A part from R A , nine other phenolic acids were detected in transform ed roots and quantified by gas chromatography.